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Figures were generated using PyMOL ( Ghrelin-bound ghrelin receptor-Go complex formation and purification The cell pellets were lysed in a buffer containing 20 mM HEPES, pH 7.5, 100 mM NaCl, and 2 mM MgCl 2 supplemented with EDTA-free protease inhibitor cocktail (Bimake) by dounce homogenization

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doi:10.1053/j.gastro.2015.05.040 84

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